Degradable Porous Gelatin Microcarrier-CellCradle

Short Delivery Time, Degradable, High Cost Performance Carriers!

CellCradle is a porous gelatin microcarriers developed and operated by Luoyang Saiao Biotechnology Co., Ltd. The microcarriers is formed from medical grade gelatin through emulsification, pore-forming, cross-linking and freeze-drying, with a surface area of approximately 9000cm²/g. CellCradle is in the form of a white flowable powder. The dry powder particle size can be customized to 50 to 500μm. It has a 20 to 30μm macroporous structure and provides a stable internal environment for cell adhesion and growth.

Application

The culture of cells difficult to adhere to walls such as mesenchymal stem cells (MSCs), Vero cell serum-free culture, as well as serum-free and low-serum culture of adherent cell such as MRC-5 and 2BS diploid Cells.

 Features  

• ◎ This type of carrier is a porous microcarrier structure. Both the interior of the carrier and the surface of the carrier can be used for cell adhesion. The adhesion area is about 9000cm²/g.

• ◎  When culture MSCs cells, the maximum cell density is more than 2 times that of classic dextran microcarriers.
• ◎  CellCradle carrier is made of medical high-grade gelatin material and can be completely degraded by trypsin and recombinant collagenase in short time. The cell harvest rate is high, and the cell harvest rate can reach more than 90%.
• ◎  It can be used for large-scale culture in the fields of cell therapy, exosome preparation, biopharmaceuticals and animal vaccines.

 Technical Parameters

Test items	Specification
Appearance	White or off-white powder, odorless and tasteless
Swelling * (ml/g dry powder)	12.0 ~ 16.0​
Buoyant density *	<1.045
Pore size ( μm )	20~50μm
Particle size distribution ( μm )	100~500μm, customizable
Degradation time ( min )	＜15
Endotoxin ( EU/mg )	＜5
Effective culture area ( cm2/g )	≥9000
Heavy metal content ( μg/g )	＜10
Cell growth (MSCs)	The culture medium is aMEM + 5% platelet lysate, 2g/L microcarrier, 100 000/ml cell density, culture for 96h, and the proliferation multiple is not less than 10 times